SequenceCollection¶
-
class
cogent3.core.alignment.
SequenceCollection
(data, names=None, alphabet=None, moltype=None, name=None, info=None, conversion_f=None, is_array=False, force_same_data=False, remove_duplicate_names=False, label_to_name=None, suppress_named_seqs=False)¶ Container for unaligned sequences
- Attributes
num_seqs
Returns the number of sequences in the alignment.
- seqs
Methods
add_seqs
(self, other[, before_name, after_name])Returns new object of class self with sequences from other added.
annotate_from_gff
(self, f)Copies annotations from gff-format file to self.
apply_pssm
(self[, pssm, path, background, …])scores sequences using the specified pssm
copy
(self)Returns deep copy of self.
copy_annotations
(self, unaligned)Copies annotations from seqs in unaligned to self, matching by name.
counts
(self[, motif_length, …])returns dict of counts of motifs
counts_per_seq
(self[, motif_length, …])returns dict of counts of motifs per sequence
deepcopy
(self[, sliced])Returns deep copy of self.
degap
(self, \*\*kwargs)Returns copy in which sequences have no gaps.
dotplot
(self[, name1, name2, window, …])make a dotplot between specified sequences.
entropy_per_seq
(self[, motif_length, …])Returns the Shannon entropy per sequence.
get_ambiguous_positions
(self)Returns dict of seq:{position:char} for ambiguous chars.
get_identical_sets
(self[, mask_degen])returns sets of names for sequences that are identical
get_lengths
(self[, include_ambiguity, allow_gap])returns {name: seq length, …}
get_motif_probs
(self[, alphabet, …])Return a dictionary of motif probs, calculated as the averaged frequency across sequences.
get_seq
(self, seqname)Return a sequence object for the specified seqname.
get_seq_indices
(self, f[, negate])Returns list of keys of seqs where f(row) is True.
get_similar
(self, target[, min_similarity, …])Returns new Alignment containing sequences similar to target.
get_translation
(self[, gc, incomplete_ok])translate from nucleic acid to protein
has_terminal_stops
(self[, gc, allow_partial])Returns True if any sequence has a terminal stop codon.
is_ragged
(self)Returns True if alignment has sequences of different lengths.
iter_selected
(self[, seq_order, pos_order])Iterates over elements in the alignment.
iter_seqs
(self[, seq_order])Iterates over values (sequences) in the alignment, in order.
omit_gap_runs
(self[, allowed_run])Returns new alignment where all seqs have runs of gaps <=allowed_run.
omit_gap_seqs
(self[, allowed_gap_frac])Returns new alignment with seqs that have <= allowed_gap_frac.
pad_seqs
(self[, pad_length])Returns copy in which sequences are padded to same length.
probs_per_seq
(self[, motif_length, …])return MotifFreqsArray per sequence
rc
(self)Returns the reverse complement alignment
rename_seqs
(self, renamer)returns new instance with sequences renamed
reverse_complement
(self)Returns the reverse complement alignment.
set_repr_policy
(self[, num_seqs, num_pos, …])specify policy for repr(self)
strand_symmetry
(self[, motif_length])returns dict of strand symmetry test results per seq
take_seqs
(self, seqs[, negate])Returns new Alignment containing only specified seqs.
take_seqs_if
(self, f[, negate])Returns new Alignment containing seqs where f(row) is True.
to_dict
(self)Returns the alignment as dict of names -> strings.
to_dna
(self)returns copy of self as an alignment of DNA moltype seqs
to_fasta
(self)Return alignment in Fasta format
to_json
(self)returns json formatted string
to_moltype
(self, moltype)returns copy of self with moltype seqs
to_nexus
(self, seq_type[, interleave_len])Return alignment in NEXUS format and mapping to sequence ids
to_phylip
(self)Return alignment in PHYLIP format and mapping to sequence ids
to_protein
(self)returns copy of self as an alignment of PROTEIN moltype seqs
to_rich_dict
(self)returns detailed content including info and moltype attributes
to_rna
(self)returns copy of self as an alignment of RNA moltype seqs
trim_stop_codons
(self[, gc, allow_partial])Removes any terminal stop codons from the sequences
with_modified_termini
(self)Changes the termini to include termini char instead of gapmotif.
write
(self[, filename, format])Write the alignment to a file, preserving order of sequences.
-
add_seqs
(self, other, before_name=None, after_name=None)¶ Returns new object of class self with sequences from other added.
- Parameters
- other
same class as self or coerceable to that class
- before_namestr
which sequence is added
- after_namestr
which sequence is added
Notes
If both before_name and after_name are specified, the seqs will be inserted using before_name.
By default the sequence is appended to the end of the alignment, this can be changed by using either before_name or after_name arguments.
-
annotate_from_gff
(self, f)¶ Copies annotations from gff-format file to self.
Matches by name of sequence. This method accepts string path or pathlib.Path or file-like object (e.g. StringIO)
Skips sequences in the file that are not in self.
-
apply_pssm
(self, pssm=None, path=None, background=None, pseudocount=0, names=None, ui=None)¶ scores sequences using the specified pssm
- Parameters
- pssmprofile.PSSM
if not provided, will be loaded from path
- path
path to either a jaspar or cisbp matrix (path must end have a suffix matching the format).
- pseudocount
adjustment for zero in matrix
- names
returns only scores for these sequences and in the name order
- Returns
- numpy array of log2 based scores at every position
-
copy
(self)¶ Returns deep copy of self.
-
copy_annotations
(self, unaligned)¶ Copies annotations from seqs in unaligned to self, matching by name.
Alignment programs like ClustalW don’t preserve annotations, so this method is available to copy annotations off the unaligned sequences.
unaligned should be a dictionary of Sequence instances.
Ignores sequences that are not in self, so safe to use on larger dict of seqs that are not in the current collection/alignment.
-
counts
(self, motif_length=1, include_ambiguity=False, allow_gap=False, exclude_unobserved=False)¶ returns dict of counts of motifs
- Parameters
- motif_length
number of elements per character.
- include_ambiguity
if True, motifs containing ambiguous characters from the seq moltype are included. No expansion of those is attempted.
- allow_gaps
if True, motifs containing a gap character are included.
- exclude_unobserved
if True, unobserved motif combinations are excluded.
Notes
only non-overlapping motifs are counted
-
counts_per_seq
(self, motif_length=1, include_ambiguity=False, allow_gap=False, exclude_unobserved=False)¶ returns dict of counts of motifs per sequence
- Parameters
- motif_length
number of characters per tuple.
- include_ambiguity
if True, motifs containing ambiguous characters from the seq moltype are included. No expansion of those is attempted.
- allow_gap
if True, motifs containing a gap character are included.
Notes
only non-overlapping motifs are counted
-
deepcopy
(self, sliced=True)¶ Returns deep copy of self.
-
degap
(self, **kwargs)¶ Returns copy in which sequences have no gaps.
-
dotplot
(self, name1=None, name2=None, window=20, threshold=None, min_gap=0, width=500, title=None, rc=False, show_progress=False)¶ make a dotplot between specified sequences. Random sequences chosen if names not provided.
- Parameters
- name1, name2str or None
names of sequences. If one is not provided, a random choice is made
- windowint
k-mer size for comparison between sequences
- thresholdint
windows where the sequences are identical >= threshold are a match
- min_gapint
permitted gap for joining adjacent line segments, default is no gap joining
- widthint
figure width. Figure height is computed based on the ratio of len(seq1) / len(seq2)
- title
title for the plot
- rcbool or None
include dotplot of reverse compliment also. Only applies to Nucleic acids moltypes
- Returns
- ——-
- a Drawable or AnnotatedDrawable
-
entropy_per_seq
(self, motif_length=1, include_ambiguity=False, allow_gap=False, exclude_unobserved=True, alert=False)¶ Returns the Shannon entropy per sequence.
- Parameters
- motif_length: int
number of characters per tuple.
- include_ambiguity: bool
if True, motifs containing ambiguous characters from the seq moltype are included. No expansion of those is attempted.
- allow_gap: bool
if True, motifs containing a gap character are included.
- exclude_unobserved: bool
if True, unobserved motif combinations are excluded.
Notes
For motif_length > 1, it’s advisable to specify exclude_unobserved=True, this avoids unnecessary calculations.
-
get_ambiguous_positions
(self)¶ Returns dict of seq:{position:char} for ambiguous chars.
Used in likelihood calculations.
-
get_identical_sets
(self, mask_degen=False)¶ returns sets of names for sequences that are identical
- Parameters
- mask_degen
if True, degenerate characters are ignored
-
get_lengths
(self, include_ambiguity=False, allow_gap=False)¶ returns {name: seq length, …}
- Parameters
- include_ambiguity
if True, motifs containing ambiguous characters from the seq moltype are included. No expansion of those is attempted.
- allow_gaps
if True, motifs containing a gap character are included.
-
get_motif_probs
(self, alphabet=None, include_ambiguity=False, exclude_unobserved=False, allow_gap=False, pseudocount=0)¶ Return a dictionary of motif probs, calculated as the averaged frequency across sequences.
- Parameters
- include_ambiguity
if True resolved ambiguous codes are included in estimation of frequencies, default is False.
- exclude_unobserved
if True, motifs that are not present in the alignment are excluded from the returned dictionary, default is False.
- allow_gap
allow gap motif
Notes
only non-overlapping motifs are counted
-
get_seq
(self, seqname)¶ Return a sequence object for the specified seqname.
-
get_seq_indices
(self, f, negate=False)¶ Returns list of keys of seqs where f(row) is True.
List will be in the same order as self.names, if present.
-
get_similar
(self, target, min_similarity=0.0, max_similarity=1.0, metric=<cogent3.util.transform.for_seq object at 0x7fcd68dd4990>, transform=None)¶ Returns new Alignment containing sequences similar to target.
- Parameters
- target
sequence object to compare to. Can be in the alignment.
- min_similarity
minimum similarity that will be kept. Default 0.0.
- max_similarity
maximum similarity that will be kept. Default 1.0. (Note that both min_similarity and max_similarity are inclusive.) metric similarity function to use. Must be f(first_seq, second_seq).
- The default metric is fraction similarity, ranging from 0.0 (0%
- identical) to 1.0 (100% identical). The Sequence classes have lots
- of methods that can be passed in as unbound methods to act as the
- metric, e.g. frac_same_gaps.
- transform
transformation function to use on the sequences before the metric is calculated. If None, uses the whole sequences in each case. A frequent transformation is a function that returns a specified range of a sequence, e.g. eliminating the ends. Note that the transform applies to both the real sequence and the target sequence.
- WARNING: if the transformation changes the type of the sequence (e.g.
- extracting a string from an RnaSequence object), distance metrics that
- depend on instance data of the original class may fail.
-
get_translation
(self, gc=None, incomplete_ok=False, **kwargs)¶ translate from nucleic acid to protein
- Parameters
- gc
genetic code, either the number or name (use cogent3.core.genetic_code.available_codes)
- incomplete_okbool
codons that are mixes of nucleotide and gaps converted to ‘?’. raises a ValueError if False
- kwargs
related to construction of the resulting object
- Returns
- A new instance of self translated into protein
-
has_terminal_stops
(self, gc=None, allow_partial=False)¶ Returns True if any sequence has a terminal stop codon.
- Parameters
- gc
genetic code object
- allow_partial
if True and the sequence length is not divisible by 3, ignores the 3’ terminal incomplete codon
-
is_array
= {'array', 'array_seqs'}¶
-
is_ragged
(self)¶ Returns True if alignment has sequences of different lengths.
-
iter_selected
(self, seq_order=None, pos_order=None)¶ Iterates over elements in the alignment.
seq_order (names) can be used to select a subset of seqs. pos_order (positions) can be used to select a subset of positions.
Always iterates along a seq first, then down a position (transposes normal order of a[i][j]; possibly, this should change)..
WARNING: Alignment.iter_selected() is not the same as alignment.iteritems() (which is the built-in dict iteritems that iterates over key-value pairs).
-
iter_seqs
(self, seq_order=None)¶ Iterates over values (sequences) in the alignment, in order.
seq_order: list of keys giving the order in which seqs will be returned. Defaults to self.Names. Note that only these sequences will be returned, and that KeyError will be raised if there are sequences in order that have been deleted from the Alignment. If self.Names is None, returns the sequences in the same order as self.named_seqs.values().
Use map(f, self.seqs()) to apply the constructor f to each seq. f must accept a single list as an argument.
Always returns references to the same objects that are values of the alignment.
-
moltype
= MolType(('\x00', '\x01', '\x02', '\x03', '\x04', '\x05', '\x06', '\x07', '\x08', '\t', '\n', '\x0b', '\x0c', '\r', '\x0e', '\x0f', '\x10', '\x11', '\x12', '\x13', '\x14', '\x15', '\x16', '\x17', '\x18', '\x19', '\x1a', '\x1b', '\x1c', '\x1d', '\x1e', '\x1f', ' ', '!', '"', '#', '$', '%', '&', "'", '(', ')', '*', '+', ',', '-', '.', '/', '0', '1', '2', '3', '4', '5', '6', '7', '8', '9', ':', ';', '<', '=', '>', '?', '@', 'A', 'B', 'C', 'D', 'E', 'F', 'G', 'H', 'I', 'J', 'K', 'L', 'M', 'N', 'O', 'P', 'Q', 'R', 'S', 'T', 'U', 'V', 'W', 'X', 'Y', 'Z', '[', '\\', ']', '^', '_', '`', 'a', 'b', 'c', 'd', 'e', 'f', 'g', 'h', 'i', 'j', 'k', 'l', 'm', 'n', 'o', 'p', 'q', 'r', 's', 't', 'u', 'v', 'w', 'x', 'y', 'z', '{', '|', '}', '~', '\x7f', '\x80', '\x81', '\x82', '\x83', '\x84', '\x85', '\x86', '\x87', '\x88', '\x89', '\x8a', '\x8b', '\x8c', '\x8d', '\x8e', '\x8f', '\x90', '\x91', '\x92', '\x93', '\x94', '\x95', '\x96', '\x97', '\x98', '\x99', '\x9a', '\x9b', '\x9c', '\x9d', '\x9e', '\x9f', '\xa0', '¡', '¢', '£', '¤', '¥', '¦', '§', '¨', '©', 'ª', '«', '¬', '\xad', '®', '¯', '°', '±', '²', '³', '´', 'µ', '¶', '·', '¸', '¹', 'º', '»', '¼', '½', '¾', '¿', 'À', 'Á', 'Â', 'Ã', 'Ä', 'Å', 'Æ', 'Ç', 'È', 'É', 'Ê', 'Ë', 'Ì', 'Í', 'Î', 'Ï', 'Ð', 'Ñ', 'Ò', 'Ó', 'Ô', 'Õ', 'Ö', '×', 'Ø', 'Ù', 'Ú', 'Û', 'Ü', 'Ý', 'Þ', 'ß', 'à', 'á', 'â', 'ã', 'ä', 'å', 'æ', 'ç', 'è', 'é', 'ê', 'ë', 'ì', 'í', 'î', 'ï', 'ð', 'ñ', 'ò', 'ó', 'ô', 'õ', 'ö', '÷', 'ø', 'ù', 'ú', 'û', 'ü', 'ý', 'þ', 'ÿ'))¶
-
property
num_seqs
¶ Returns the number of sequences in the alignment.
-
omit_gap_runs
(self, allowed_run=1)¶ Returns new alignment where all seqs have runs of gaps <=allowed_run.
Note that seqs with exactly allowed_run gaps are not deleted. Default is for allowed_run to be 1 (i.e. no consecutive gaps allowed).
Because the test for whether the current gap run exceeds the maximum allowed gap run is only triggered when there is at least one gap, even negative values for allowed_run will still let sequences with no gaps through.
-
omit_gap_seqs
(self, allowed_gap_frac=0)¶ Returns new alignment with seqs that have <= allowed_gap_frac.
allowed_gap_frac should be a fraction between 0 and 1 inclusive. Default is 0.
-
pad_seqs
(self, pad_length=None, **kwargs)¶ Returns copy in which sequences are padded to same length.
- Parameters
- pad_length
Length all sequences are to be padded to. Will pad to max sequence length if pad_length is None or less than max length.
-
probs_per_seq
(self, motif_length=1, include_ambiguity=False, allow_gap=False, exclude_unobserved=False, alert=False)¶ return MotifFreqsArray per sequence
-
rc
(self)¶ Returns the reverse complement alignment
-
rename_seqs
(self, renamer)¶ returns new instance with sequences renamed
- Parameters
- renamercallable
function that will take current sequences and return the new one
-
reverse_complement
(self)¶ Returns the reverse complement alignment. A synonymn for rc.
-
property
seqs
¶
-
set_repr_policy
(self, num_seqs=None, num_pos=None, ref_name=None)¶ specify policy for repr(self)
- Parameters
- num_seqsint or None
number of sequences to include in represented display.
- num_posint or None
length of sequences to include in represented display.
- ref_namestr or None
name of sequence to be placed first, or “longest” (default). If latter, indicates longest sequence will be chosen.
-
strand_symmetry
(self, motif_length=1)¶ returns dict of strand symmetry test results per seq
-
take_seqs
(self, seqs, negate=False, **kwargs)¶ Returns new Alignment containing only specified seqs.
Note that the seqs in the new alignment will be references to the same objects as the seqs in the old alignment.
-
take_seqs_if
(self, f, negate=False, **kwargs)¶ Returns new Alignment containing seqs where f(row) is True.
Note that the seqs in the new Alignment are the same objects as the seqs in the old Alignment, not copies.
-
to_dict
(self)¶ Returns the alignment as dict of names -> strings.
Note: returns strings, NOT Sequence objects.
-
to_dna
(self)¶ returns copy of self as an alignment of DNA moltype seqs
-
to_fasta
(self)¶ Return alignment in Fasta format
- Parameters
- make_seqlabel
callback function that takes the seq object and returns a label str
-
to_json
(self)¶ returns json formatted string
-
to_moltype
(self, moltype)¶ returns copy of self with moltype seqs
-
to_nexus
(self, seq_type, interleave_len=50)¶ Return alignment in NEXUS format and mapping to sequence ids
- NOTE Not that every sequence in the alignment MUST come from
a different species!! (You can concatenate multiple sequences from same species together before building tree)
seq_type: dna, rna, or protein
Raises exception if invalid alignment
-
to_phylip
(self)¶ Return alignment in PHYLIP format and mapping to sequence ids
raises exception if invalid alignment
-
to_protein
(self)¶ returns copy of self as an alignment of PROTEIN moltype seqs
-
to_rich_dict
(self)¶ returns detailed content including info and moltype attributes
-
to_rna
(self)¶ returns copy of self as an alignment of RNA moltype seqs
-
trim_stop_codons
(self, gc=None, allow_partial=False, **kwargs)¶ Removes any terminal stop codons from the sequences
- Parameters
- gc
genetic code object
- allow_partial
if True and the sequence length is not divisible by 3, ignores the 3’ terminal incomplete codon
-
with_modified_termini
(self)¶ Changes the termini to include termini char instead of gapmotif.
Useful to correct the standard gap char output by most alignment programs when aligned sequences have different ends.
-
write
(self, filename=None, format=None, **kwargs)¶ Write the alignment to a file, preserving order of sequences.
- Parameters
- filename
name of the sequence file
- format
format of the sequence file
Notes
If format is None, will attempt to infer format from the filename suffix.